Density gradient centrifugation prior to cryopreservation and hypotaurine supplementation improve post-thaw quality of sperm from infertile men with oligoasthenoteratozoospermia
نویسندگان
چکیده
منابع مشابه
Sperm selection using single layer centrifugation prior to cryopreservation can increase thawed sperm quality in stallions.
REASONS FOR PERFORMING STUDY The increasing use of modern reproductive techniques in human medicine has led to a higher demand for isolation of motile sperm. Several of these isolation techniques have been adapted for veterinary use and can be applied for the selection of a superior sperm sample from stallion semen. Until recently a major disadvantage of such isolation techniques was the limita...
متن کاملComparison of Sperm Telomere Length between Two Sperm Selection Procedures: Density Gradient Centrifugation and Zeta Potential
Background: Telomeres are particular sequences of DNA located at the end of the eukaryotic chromosomes that are essential for genome integrity. Telomere length in spermatozoa differs among males, as well as spermatozoa. Also, decreased telomere length in spermatozoa of infertile men is associated with the reduction of fertility potential and embryo quality. Density gradient centrifugation (DGC)...
متن کاملThe post - thaw quality of ram sperm held for 0 to 48 h at 5 ◦ C prior to cryopreservation
The effects of holding diluted ram semen at 5 ◦C for up to 48 h prior to cryopreservation were investigated. Semen from six rams was collected by electro-ejaculation in the autumn and again from six different rams in the spring. The sperm concentration and motility were determined using spectrophotometry and computerized automated semen analysis, respectively. Samples were diluted at 23 ◦C to 4...
متن کاملEffects of Centrifugation and Different Levels of Soybean Lecithin-Based Extender on Post-Thaw Sperm Quality of Ghezel Ram Semen
The objective of this study was to specify the effects of centrifugation and different surface of soybean lecithin-based extender on post-thaw Sperm quality of Ghezel ram semen. Semen samples were gathered from 5 mature Ghezel ram two/week for 3 weeks. After initial evaluation, the approved semen samples were pooled together and split into equal parts in Falcon tubes. Three samples were combine...
متن کاملcan zeta sperm selection method, recover sperm with higher dna integrity compare to density gradient centrifugation?
background: sperm selection for icsi based on morphology and motility might not be relevant to chromatin integrity. thus sperm selection based on sperm characteristics has been suggested. objective: the aim of this study was to compare the efficiency of zeta method with routine density gradient centrifugation method (dgc) for the selection of sperm with higher dna integrity. materials and metho...
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ژورنال
عنوان ژورنال: Human Reproduction
سال: 2013
ISSN: 0268-1161,1460-2350
DOI: 10.1093/humrep/det253